Abstract
The violet and purple colored fruits contain phenolic compounds specifically, anthocyanins. These have a high nutritional quality and anthocyanins are believed to have anti-aging, anti-carcinogenic and antioxidant properties in the fruit. Gene expression studies have demonstrated the importance of flavonoids and their biosynthetic pathways, further increasing the interests of research and sources. In the present study, for the isolation and quantification of the β-Actin, RuANS and RuMYB10 genes involved in the expression of anthocyanins in the cascade of Rubus glaucus Benth, the Real-Time Polymerase Chain Reaction Reverse transcription (RT-qPCR). According to the values obtained with the statistical analyzes DCA and the Turkey test at 95% confidence, observed a relationship between the genes as no significant differences existed in the expression of the three, generating the following results: Actin presented higher concentration (273.42ng/mL), followed by the RuMYB10 gene (220.33ng/mL) and finally the Ruans gene (212.75ng/mL), concluding that the species studied had similar levels of expression Three genes analyzed.
Translated title of the contribution | Aislamiento y Cuantificación de Tres Genes Involucrados en la Expresión de Antocianinas en Rubus Glaucus Benth por Rt-qpcr |
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Original language | English (US) |
Pages (from-to) | 314-316 |
Number of pages | 3 |
Journal | Moj Food Processing & Technology |
Volume | 5 |
Issue number | 5 |
DOIs | |
State | Published - 8 Dec 2017 |
Keywords
- Anthocyanins
- Rt-qpcr
- Ruans gene
- Rubus glaucus benth
- Rumyb10 gene
- Β-actin gene
CACES Knowledge Areas
- 215A Biochemistry